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Power cycle the unit. If the error returns upon restarting, the internal speed sensor must be replaced by a technician. 6. Emergency Lid Release Procedure
No rotor detected; ensure the rotor is properly seated and secured .
What are you experiencing with your unit?
The standard 24-place fixed-angle rotor is autoclavable at 121°C for 20 minutes if biological decontamination is necessary. Material Compatibility Warnings
For temperature-sensitive work, use the function. This feature can cool the rotor chamber from ambient room temperature down to 4°C in approximately 10 minutes . 2. Loading the Rotor EPPENDORF - 5402 Refrigerated Microcentrifuge - LabWrench eppendorf 5402 manual updated
Verify that the rotor is securely fastened with the rotor nut. Never operate the centrifuge without a properly tightened rotor. 2.2 Loading the Rotor
Disclaimer: This guide is a summary of typical operations. Always refer to the original manufacturer's documentation accompanying your specific unit for the most accurate information.
In the event of a power outage or a system error, your samples can be retrieved manually.
Operating at sub-ambient temperatures causes ice buildup on the chamber walls. Switch off the centrifuge and unplug the power cord. Leave the lid open to let the ice melt naturally. Soak up the melted water with a cloth. Wipe the chamber dry before restarting the unit. 5. Updated Troubleshooting and Error Codes Power cycle the unit
Ensure the rotor chamber is clean, dry, and free of debris or ice buildup. Loading and Balancing the Rotor (Critical Safety Step)
Note: The Eppendorf 5402 is a legacy, discontinued product. For information regarding parts and specialized technical service, it is advisable to contact qualified third-party laboratory equipment service providers familiar with this model.
Available in 120V (60 Hz) and 230V (50-60 Hz) configurations 2. Interface and Control Panel Overview
| Error Code(s) | Likely Cause(s) | Recommended Troubleshooting Steps | | :--- | :--- | :--- | | | Rotor imbalance or over-speed detection; rotor sensor issue | Verify rotor is seated/secured; ensure tubes are balanced; inspect for damage/corrosion; wait 15-30 min to cool; check correct rotor type in settings. | | "balan." error | Rotor imbalance, often at low speeds (e.g., >700 rpm) | Ensure tubes and adapters are symmetrically loaded and of equal weight; rebalance all tubes before running. | | ERROR 1 | Not a balance error but an issue with the power supply or internal electronics | Check power cord and wall outlet; if problem persists, internal electronics may require professional service. | | Error Code A | Temperature sensor malfunction | Check sensor connections. As the original part may be discontinued, professional diagnosis is required. | | E2 | Speed too low; may be due to sample imbalance or a loose rotor | Check sample balance; ensure the rotor is correctly installed and tightened. | | E7 (Temp Sensor Fault) | Temperature sensor or its wiring is faulty | Inspect the temperature sensor connection. Persistent errors indicate a need for replacement by a technician. | Emergency Lid Release Procedure No rotor detected; ensure
Running the centrifuge at 4°C or lower causes humidity in the air to freeze along the chamber walls.
If you need the original for replacement pieces
includes a fast-cool function that can lower the chamber temperature from ambient to 4°C in approximately 10 minutes.
Samples must be loaded symmetrically in pairs opposite each other.