Samples must always be loaded symmetrically to avoid excessive vibration and damage to the motor.
This guide acts as a comprehensive summary of the , providing essential information on installation, operation, safety, and maintenance to ensure optimal performance and longevity of your centrifuge. 1. Introduction and Technical Specifications
The Eppendorf 5402 is a benchtop refrigerated microcentrifuge designed for routine and research applications. It is known for its versatility, capable of performing as a high-capacity general-purpose centrifuge for cell harvesting, a high-speed centrifuge for cell lysate separation, and a microcentrifuge for DNA precipitation. A key feature is its built-in refrigeration, allowing precise temperature control for temperature-sensitive samples.
To ensure safe and effective operation of the Eppendorf 5402 centrifuge, follow these general guidelines: eppendorf 5402 manual
The Eppendorf 5402 is characterized by its digital controls and precise cooling capabilities. Specification 14,000 RPM (up to 16,000 x g) Speed Increments Temperature Range -9°C to 40°C Timer 0 to 99 minutes or continuous (momentary button) Capacity Standard 18-place rotor for 1.5/2.0 mL tubes Dimensions 60 cm (W) x 47 cm (D) x 29 cm (H) Weight Approximately 43 kg (95 lbs) Key Operating Instructions
Regular maintenance ensures the centrifuge remains safe and accurate.
To gather this information, I will perform multiple searches to cover different aspects: general information about the centrifuge, the manual itself, specifications, common issues, and perhaps user experiences. I'll also look for official sources from Eppendorf and manuals from third-party sites. search results show various sources. Manuals are available on sites like Manualzz, Manualsdir, and Manualmachine. There's also a Labwrench page with features and a Fixya page about an error. I should open these to gather detailed information. I'll also open the Eppendorf Japan page as it might have legacy product information. opened pages provide useful information. The Manualzz page includes key features, loading instructions, and maintenance steps. The Labwrench page shows features and specifications. The Fixya page discusses error 02. The Eppendorf PDF shows that the 5402 is discontinued, with successors 5418 R and 5425 R. I should also search for "Eppendorf 5402 service manual" and "Eppendorf 5402 parts". search results provide additional information. The American Instrument page mentions a brushless motor and rotor identification. I should also search for "Eppendorf 5402 manual PDF free"., I need to structure a long article. The outline could include: introduction, overview of Eppendorf 5402, where to find the manual, technical specifications, safety guidelines, operating instructions, maintenance, troubleshooting, and conclusion. I'll also need to cite sources. I'll also search for "Eppendorf 5402 error codes list"., I'll gather information on the F-45-18-11 rotor, as it's commonly used with the 5402. I'll search for "F-45-18-11 manual". have gathered sufficient information. The article will cover: introduction, technical specifications, safety guidelines, operating instructions, maintenance, troubleshooting, rotor compatibility, and how to obtain the manual. I will use the information from Manualzz, Labwrench, Fixya, Eppendorf PDF, and other sources. I will cite them accordingly. Now I will proceed to write the article.ppendorf 5402 Refrigerated Microcentrifuge: The Essential User Manual and Maintenance Guide Samples must always be loaded symmetrically to avoid
To ensure safety and equipment longevity according to standard Eppendorf safety protocols:
The rotor is still spinning, or the electronic latch has failed.
This is the workhorse rotor for the 5402, suitable for most routine applications. To ensure safe and effective operation of the
Stop run immediately. Balance tubes perfectly and tighten the rotor lid. Power failure or active rotor spinning.
Eppendorf 5402 is a legacy refrigerated microcentrifuge designed for temperature-sensitive laboratory work, offering a speed range up to 14,000 RPM (~16,000 x g) and a temperature control range from -9°C to 40°C Key Features & Operation Based on manual specifications for this model: Typically accommodates up to 18 x 1.5/2.0 mL tubes Fast Cool Function: Can cool the rotor chamber from ambient temperature to 4°C in approximately 10 minutes